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KMID : 0386520140230010093
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Research Institute of Korean Medicone
2014 Volume.23 No. 1 p.93 ~ p.104
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Quantitative Analysis of the Marker Components in Glycyrrhizae Radix et Rhizoma by Processing Method
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Seo Chang-Seob
Kim Jung-Hoon
Shin Hyeun-Kyoo
Hwang Seok-Youn
Kim Byoung-Soo
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Abstract
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Glycyrrhizae Radix et Rhizoma has been extensively used by human beings as a medicinal herb as well as natural sweetener. In this study, we performed quantification analysis of three major constituents including liquiritin, glycyrrhizin, and glycyrrhetinic acid in the 70% ethanol extracts of non-processed Glycyrrhizae Radix et Rhizoma and processed Glycyrrhizae Radix et Rhizoma using a high-performance liquid chromatography coupled with photodiode array detector. The analytical column for separation of the 3 constituents used a Gemini C18 column kept at 40°C by the gradient elution of two mobile phase. The amounts of liquiritin, glycyrrhizin, and glycyrrhetinic acid in non-processed Glycyrrhizae Radix et Rhizoma were 2.57%, 3.52%, and not detected. After processing by roasting, the best roasting temperature and time of iquiritin, glycyrrhizin, and glycyrrhetinic acid were 160℃-15 min (2.46%), 160℃-15 min (3.67%), and 240℃-15 min (0.76%),
respectively.
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KEYWORD
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Glycyrrhizae Radix et Rhizoma , Pocessing Method , Liquiritin , Liquiritigenin , Glycyrrhizin , Glycyrrhrtic acid
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